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control fibroblasts ndhf  (Lonza)


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    Lonza control fibroblasts ndhf
    Oxygen consumption analysis: Oxygen consumption was evaluated using the Seahorse XF Analyzer; a Oxygen consumption rate (OCR) expressed as percent (%) of rate measurement 13 in control cells <t>(NDHF)</t> and in control cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1, +AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); b OCR expressed as % of rate measurement 13 in NDHF, in NDUFS4-deficient cells (NDUFS4) and in patient cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1,+AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); c Maximal respiration rate in CI deficient cells (carrying pathogenic variants in ACAD9 and NDUFS4), before and after transduction with AtNDA1, AtNDA2, AtNDB4 and MTS-ScNDI1. Values were normalized to maximal respiration of untransduced control cells; d Oxygen consumption rate (OCR) expressed as pmol O 2 /min/cell in cell lines presenting with CI-defect due to mutations in ACAD9, NDUFB3, NDUFS4, ND5, tRNA Leu before and after transduction with alternative dehydrogenases from A. thaliana and yeast. Each cell line was measured at least twice in independent experiments. During experiment, four technical replicates were run for each cell line. Values are expressed as mean ± SD
    Control Fibroblasts Ndhf, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control fibroblasts ndhf/product/Lonza
    Average 90 stars, based on 1 article reviews
    control fibroblasts ndhf - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Arabidopsis thaliana alternative dehydrogenases: a potential therapy for mitochondrial complex I deficiency? Perspectives and pitfalls"

    Article Title: Arabidopsis thaliana alternative dehydrogenases: a potential therapy for mitochondrial complex I deficiency? Perspectives and pitfalls

    Journal: Orphanet Journal of Rare Diseases

    doi: 10.1186/s13023-019-1185-3

    Oxygen consumption analysis: Oxygen consumption was evaluated using the Seahorse XF Analyzer; a Oxygen consumption rate (OCR) expressed as percent (%) of rate measurement 13 in control cells (NDHF) and in control cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1, +AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); b OCR expressed as % of rate measurement 13 in NDHF, in NDUFS4-deficient cells (NDUFS4) and in patient cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1,+AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); c Maximal respiration rate in CI deficient cells (carrying pathogenic variants in ACAD9 and NDUFS4), before and after transduction with AtNDA1, AtNDA2, AtNDB4 and MTS-ScNDI1. Values were normalized to maximal respiration of untransduced control cells; d Oxygen consumption rate (OCR) expressed as pmol O 2 /min/cell in cell lines presenting with CI-defect due to mutations in ACAD9, NDUFB3, NDUFS4, ND5, tRNA Leu before and after transduction with alternative dehydrogenases from A. thaliana and yeast. Each cell line was measured at least twice in independent experiments. During experiment, four technical replicates were run for each cell line. Values are expressed as mean ± SD
    Figure Legend Snippet: Oxygen consumption analysis: Oxygen consumption was evaluated using the Seahorse XF Analyzer; a Oxygen consumption rate (OCR) expressed as percent (%) of rate measurement 13 in control cells (NDHF) and in control cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1, +AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); b OCR expressed as % of rate measurement 13 in NDHF, in NDUFS4-deficient cells (NDUFS4) and in patient cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1,+AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); c Maximal respiration rate in CI deficient cells (carrying pathogenic variants in ACAD9 and NDUFS4), before and after transduction with AtNDA1, AtNDA2, AtNDB4 and MTS-ScNDI1. Values were normalized to maximal respiration of untransduced control cells; d Oxygen consumption rate (OCR) expressed as pmol O 2 /min/cell in cell lines presenting with CI-defect due to mutations in ACAD9, NDUFB3, NDUFS4, ND5, tRNA Leu before and after transduction with alternative dehydrogenases from A. thaliana and yeast. Each cell line was measured at least twice in independent experiments. During experiment, four technical replicates were run for each cell line. Values are expressed as mean ± SD

    Techniques Used: Control, Transduction

    Assessment of SOD activity: Spectrophotometric assessment of SOD activity according to the pyrogallol autoxidation method. NDUFS4 mutated cell line (79787) displays higher SOD activity when compared to control fibroblasts (NHDF). Transfection with AtNDB4 and AtNDA2 (79787-AtNDB4 and 79,787-AtNDA2) significantly decreases SOD activity, which is nearly restored to normal levels. Values are expressed as means ± SD (ns: not significant; *p < 0.05; **p < 0.01 )
    Figure Legend Snippet: Assessment of SOD activity: Spectrophotometric assessment of SOD activity according to the pyrogallol autoxidation method. NDUFS4 mutated cell line (79787) displays higher SOD activity when compared to control fibroblasts (NHDF). Transfection with AtNDB4 and AtNDA2 (79787-AtNDB4 and 79,787-AtNDA2) significantly decreases SOD activity, which is nearly restored to normal levels. Values are expressed as means ± SD (ns: not significant; *p < 0.05; **p < 0.01 )

    Techniques Used: Activity Assay, Control, Transfection


    Figure Legend Snippet: Expression level of AtNDA2, AtNDB4, and NADH:quinone oxidoreductase (CI) subunits in control cell lines (NDHF) before and after transduction with AtNDA2 and AtNDB4

    Techniques Used: Expressing, Control, Transduction

    Evaluation of mitochondrial respiration: Mitochondrial membrane potential variations assessed by rhodamine 123 fluorescence and oxygen uptake measured with optode device in digitonine permeabilized fibroblasts (representative graphs for the control fibroblasts - left panel and AtNDA2-transfected control fibroblasts - right panel). The reaction was started by the addition of glutamate/malate, followed by injections of ADP (see text). Note that the amount of oxygen reduced during ADP phosphorylation is significantly higher in AtNDA2 transfected cells comparing to control
    Figure Legend Snippet: Evaluation of mitochondrial respiration: Mitochondrial membrane potential variations assessed by rhodamine 123 fluorescence and oxygen uptake measured with optode device in digitonine permeabilized fibroblasts (representative graphs for the control fibroblasts - left panel and AtNDA2-transfected control fibroblasts - right panel). The reaction was started by the addition of glutamate/malate, followed by injections of ADP (see text). Note that the amount of oxygen reduced during ADP phosphorylation is significantly higher in AtNDA2 transfected cells comparing to control

    Techniques Used: Membrane, Fluorescence, Control, Transfection, Phospho-proteomics



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    control fibroblasts ndhf  (Lonza)
    90
    Lonza control fibroblasts ndhf
    Oxygen consumption analysis: Oxygen consumption was evaluated using the Seahorse XF Analyzer; a Oxygen consumption rate (OCR) expressed as percent (%) of rate measurement 13 in control cells <t>(NDHF)</t> and in control cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1, +AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); b OCR expressed as % of rate measurement 13 in NDHF, in NDUFS4-deficient cells (NDUFS4) and in patient cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1,+AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); c Maximal respiration rate in CI deficient cells (carrying pathogenic variants in ACAD9 and NDUFS4), before and after transduction with AtNDA1, AtNDA2, AtNDB4 and MTS-ScNDI1. Values were normalized to maximal respiration of untransduced control cells; d Oxygen consumption rate (OCR) expressed as pmol O 2 /min/cell in cell lines presenting with CI-defect due to mutations in ACAD9, NDUFB3, NDUFS4, ND5, tRNA Leu before and after transduction with alternative dehydrogenases from A. thaliana and yeast. Each cell line was measured at least twice in independent experiments. During experiment, four technical replicates were run for each cell line. Values are expressed as mean ± SD
    Control Fibroblasts Ndhf, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control fibroblasts ndhf/product/Lonza
    Average 90 stars, based on 1 article reviews
    control fibroblasts ndhf - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    Oxygen consumption analysis: Oxygen consumption was evaluated using the Seahorse XF Analyzer; a Oxygen consumption rate (OCR) expressed as percent (%) of rate measurement 13 in control cells (NDHF) and in control cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1, +AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); b OCR expressed as % of rate measurement 13 in NDHF, in NDUFS4-deficient cells (NDUFS4) and in patient cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1,+AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); c Maximal respiration rate in CI deficient cells (carrying pathogenic variants in ACAD9 and NDUFS4), before and after transduction with AtNDA1, AtNDA2, AtNDB4 and MTS-ScNDI1. Values were normalized to maximal respiration of untransduced control cells; d Oxygen consumption rate (OCR) expressed as pmol O 2 /min/cell in cell lines presenting with CI-defect due to mutations in ACAD9, NDUFB3, NDUFS4, ND5, tRNA Leu before and after transduction with alternative dehydrogenases from A. thaliana and yeast. Each cell line was measured at least twice in independent experiments. During experiment, four technical replicates were run for each cell line. Values are expressed as mean ± SD

    Journal: Orphanet Journal of Rare Diseases

    Article Title: Arabidopsis thaliana alternative dehydrogenases: a potential therapy for mitochondrial complex I deficiency? Perspectives and pitfalls

    doi: 10.1186/s13023-019-1185-3

    Figure Lengend Snippet: Oxygen consumption analysis: Oxygen consumption was evaluated using the Seahorse XF Analyzer; a Oxygen consumption rate (OCR) expressed as percent (%) of rate measurement 13 in control cells (NDHF) and in control cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1, +AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); b OCR expressed as % of rate measurement 13 in NDHF, in NDUFS4-deficient cells (NDUFS4) and in patient cells transduced with alternative dehydrogenases from A. thaliana (+AtNDA1,+AtNDA2, +AtNDB4) and yeast (+MTS-ScNDI1); c Maximal respiration rate in CI deficient cells (carrying pathogenic variants in ACAD9 and NDUFS4), before and after transduction with AtNDA1, AtNDA2, AtNDB4 and MTS-ScNDI1. Values were normalized to maximal respiration of untransduced control cells; d Oxygen consumption rate (OCR) expressed as pmol O 2 /min/cell in cell lines presenting with CI-defect due to mutations in ACAD9, NDUFB3, NDUFS4, ND5, tRNA Leu before and after transduction with alternative dehydrogenases from A. thaliana and yeast. Each cell line was measured at least twice in independent experiments. During experiment, four technical replicates were run for each cell line. Values are expressed as mean ± SD

    Article Snippet: The control fibroblasts (NDHF) were purchased from Lonza (Cat. No. CC-2509).

    Techniques: Control, Transduction

    Assessment of SOD activity: Spectrophotometric assessment of SOD activity according to the pyrogallol autoxidation method. NDUFS4 mutated cell line (79787) displays higher SOD activity when compared to control fibroblasts (NHDF). Transfection with AtNDB4 and AtNDA2 (79787-AtNDB4 and 79,787-AtNDA2) significantly decreases SOD activity, which is nearly restored to normal levels. Values are expressed as means ± SD (ns: not significant; *p < 0.05; **p < 0.01 )

    Journal: Orphanet Journal of Rare Diseases

    Article Title: Arabidopsis thaliana alternative dehydrogenases: a potential therapy for mitochondrial complex I deficiency? Perspectives and pitfalls

    doi: 10.1186/s13023-019-1185-3

    Figure Lengend Snippet: Assessment of SOD activity: Spectrophotometric assessment of SOD activity according to the pyrogallol autoxidation method. NDUFS4 mutated cell line (79787) displays higher SOD activity when compared to control fibroblasts (NHDF). Transfection with AtNDB4 and AtNDA2 (79787-AtNDB4 and 79,787-AtNDA2) significantly decreases SOD activity, which is nearly restored to normal levels. Values are expressed as means ± SD (ns: not significant; *p < 0.05; **p < 0.01 )

    Article Snippet: The control fibroblasts (NDHF) were purchased from Lonza (Cat. No. CC-2509).

    Techniques: Activity Assay, Control, Transfection

    Journal: Orphanet Journal of Rare Diseases

    Article Title: Arabidopsis thaliana alternative dehydrogenases: a potential therapy for mitochondrial complex I deficiency? Perspectives and pitfalls

    doi: 10.1186/s13023-019-1185-3

    Figure Lengend Snippet: Expression level of AtNDA2, AtNDB4, and NADH:quinone oxidoreductase (CI) subunits in control cell lines (NDHF) before and after transduction with AtNDA2 and AtNDB4

    Article Snippet: The control fibroblasts (NDHF) were purchased from Lonza (Cat. No. CC-2509).

    Techniques: Expressing, Control, Transduction

    Evaluation of mitochondrial respiration: Mitochondrial membrane potential variations assessed by rhodamine 123 fluorescence and oxygen uptake measured with optode device in digitonine permeabilized fibroblasts (representative graphs for the control fibroblasts - left panel and AtNDA2-transfected control fibroblasts - right panel). The reaction was started by the addition of glutamate/malate, followed by injections of ADP (see text). Note that the amount of oxygen reduced during ADP phosphorylation is significantly higher in AtNDA2 transfected cells comparing to control

    Journal: Orphanet Journal of Rare Diseases

    Article Title: Arabidopsis thaliana alternative dehydrogenases: a potential therapy for mitochondrial complex I deficiency? Perspectives and pitfalls

    doi: 10.1186/s13023-019-1185-3

    Figure Lengend Snippet: Evaluation of mitochondrial respiration: Mitochondrial membrane potential variations assessed by rhodamine 123 fluorescence and oxygen uptake measured with optode device in digitonine permeabilized fibroblasts (representative graphs for the control fibroblasts - left panel and AtNDA2-transfected control fibroblasts - right panel). The reaction was started by the addition of glutamate/malate, followed by injections of ADP (see text). Note that the amount of oxygen reduced during ADP phosphorylation is significantly higher in AtNDA2 transfected cells comparing to control

    Article Snippet: The control fibroblasts (NDHF) were purchased from Lonza (Cat. No. CC-2509).

    Techniques: Membrane, Fluorescence, Control, Transfection, Phospho-proteomics